Western blot analysis of extracts of Hela cells, using Phospho-Tau-Ser202/Thr205 antibody (AP0894) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-Tau-Ser202/Thr205 Rabbit pAb
Catalog No:AB-AP0894
Manufacturer:ABclonal
Synonyms:DDPAC;FTDP-17;MAPTL;MSTD;MTBT1;MTBT2;PPND;PPP1R103;TAU;MAPT;Tau
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Tau-Ser202/Thr205 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer’s disease, Pick’s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Ser202/Thr205 of human Tau |
Gene ID: | 4137 |
Swiss prot: | P10636 |
Molecular weight: | 32-45kDa/78- 80kDa |
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