Western blot analysis of extracts of 293T cells, using Phospho-PERK-T982 Rabbit pAb (AP0886) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-PERK-T982 Rabbit pAb
Catalog No:AB-AP0886
Manufacturer:ABclonal
Synonyms:EIF2AK3;PEK;PERK;WRS
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-PERK-T982 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T982 of human PERK. |
Gene ID: | 9451 |
Swiss prot: | Q9NZJ5 |
Molecular weight: | 125kDa |
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