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Western blot analysis of extracts of MCF7 cells, using Phospho-TSC2-T1462 antibody (AP0866) at 1:1000 dilution.MCF7 cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-TSC2-T1462 Rabbit pAb
Catalog No：AB-AP0866
Manufacturer：ABclonal
Synonyms：LAM;PPP1R160;TSC4;TSC2;Tuberin;tuberin
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-TSC2-T1462 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen: | A synthetic phosphorylated peptide around T1462 of human TSC2 (NP_000539.2). |
| Gene ID: | 7249 |
| Swiss prot: | P49815 |
| Molecular weight: | 25kDa/187-200kDa |
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