.jpg)
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S392 antibody (AP0860) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 1s.
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Western blot analysis of extracts of various cell lines, using Phospho-p53-S392 antibody (AP0860) at 1:1000 dilution.HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 20s.
Phospho-p53-S392 Rabbit pAb
Catalog No：AB-AP0860
Manufacturer：ABclonal
Synonyms：BCC7;LFS1;P53;TRP53;p53;TP53;BMFS5
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p53-S392 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). |
| Immunogen: | A synthetic phosphorylated peptide around S392 of human p53 (NP_000537.3). |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 23-43kDa |
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