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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody (AP0853) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-HSL-S660 Rabbit pAb
Catalog No：AB-AP0853
Manufacturer：ABclonal
Synonyms：LIPE;AOMS4;FPLD6;HSL;LHS
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-HSL-S660 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| Immunogen: | A synthetic phosphorylated peptide around S660 of human LIPE (NP_005348.2). |
| Gene ID: | 3991 |
| Swiss prot: | Q05469 |
| Molecular weight: | 84kDa/116kDa |
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