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Western blot analysis of extracts of various cell lines, using Phospho-HSL-S563 antibody (AP0851) at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.H9C2 cells were treated with Isoproterenol (10 μM for 20 min) at 37℃.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-HSL-S563 Rabbit pAb
Catalog No：AB-AP0851
Manufacturer：ABclonal
Synonyms：LIPE;AOMS4;FPLD6;HSL;LHS
Applications：WB
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-HSL-S563 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S853 of human HSL (NP_005348.2 ,equivalent to S563 of rat HSL |
| Gene ID: | 3991 |
| Swiss prot: | Q05469 |
| Molecular weight: | 84kDa/116kDa |
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