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Western blot analysis of extracts of HeLa cells, using Phospho-Smad1(Ser463/465)/Smad5(Ser463/465)/Smad9(Ser465/467) pAb (AP0850) at 1:1000 dilution.HeLa cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467 Rabbit pAb
Catalog No：AB-AP0850
Manufacturer：ABclonal
Synonyms：Smad1/Smad5/Smad9
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S463 of human Smad1 |
| Gene ID: | 4086/4090/4093 |
| Swiss prot: | Q15797/Q99717/O15198 |
| Molecular weight: | 60kDa |
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