Western blot analysis of extracts of various cell lines, using Phospho-SF3B1-T313 antibody (AP0844) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-SF3B1-T313 Rabbit pAb
Catalog No:AB-AP0844
Manufacturer:ABclonal
Synonyms:SF3B1;Hsh155;MDS;PRP10;PRPF10;SAP155;SF3b155
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-SF3B1-T313 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron’s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Immunogen: | A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2). |
Gene ID: | 23451 |
Swiss prot: | O75533 |
Molecular weight: | 16kDa/145kDa |
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