Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Histone H3-S10 Rabbit pAb
Catalog No:AB-AP0840
Manufacturer:ABclonal
Synonyms:H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Histone H3-S10 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
Immunogen: | A synthetic phosphorylated peptide around S10 of human Histone H3 (NP_003484.1). |
Gene ID: | 8290 |
Swiss prot: | Q16695 |
Molecular weight: | 15kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-AP0026
Manufacturer:ABclonal
Synonyms:EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0024
Manufacturer:ABclonal
Synonyms:EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0017
Manufacturer:ABclonal
Synonyms:CHEK1;CHK1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0015
Manufacturer:ABclonal
Synonyms:CDK1;CDC2;CDC28A;P34CDC2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0011
Manufacturer:ABclonal
Synonyms:BAD;BBC2;BCL2L8;Bad
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0006
Manufacturer:ABclonal
Synonyms:AAA;ABETA;ABPP;AD1;APPI;CTFgamma;CVAP;PN-II;PN2;Amyloid beta A4;APP;Aβ42;preA4
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00