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Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at dilution of 1:2000 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at dilution of 1:2000 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at dilution of 1:2000 (40x lens).
Phospho-Histone H3-S10 Rabbit pAb
Catalog No：AB-AP0840
Manufacturer：ABclonal
Synonyms：H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Histone H3-S10 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic phosphorylated peptide around S10 of human Histone H3 (NP_003484.1). |
| Gene ID: | 8290 |
| Swiss prot: | Q16695 |
| Molecular weight: | 15kDa |
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