Western blot analysis of extracts of HeLa cells, using Phospho-SRF-S103 antibody (AP0829) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-SRF-S103 Rabbit pAb
Catalog No:AB-AP0829
Manufacturer:ABclonal
Synonyms:SRF;MCM1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-SRF-S103 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic phosphorylated peptide around S103 of human SRF (NP_003122.1). |
Gene ID: | 6722 |
Swiss prot: | P11831 |
Molecular weight: | 51kDa |
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