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Western blot analysis of extracts of MCF7 cells, using Phospho-POLR2A-S5 antibody (AP0828) at 1:1000 dilution. MCF7 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunofluorescence analysis of MCF7 cells using Phospho-POLR2A-S5 antibody (AP0828) at dilution of 1:100. MCF7 cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours (left). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat testis using Phospho-POLR2A-S5 antibody (AP0828) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using Phospho-POLR2A-S5 antibody (AP0828) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Phospho-POLR2A-S5 antibody (AP0828) at dilution of 1:100 (40x lens).
Phospho-POLR2A-S5 Rabbit pAb
Catalog No：AB-AP0828
Manufacturer：ABclonal
Synonyms：POLR2A;POLR2;POLRA;RPB1;RPBh1;RPO2;RPOL2;RpIILS;hRPB220;hsRPB1;Pol II
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-POLR2A-S5 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A |
| Gene ID: | 5430 |
| Swiss prot: | P24928 |
| Molecular weight: | 63kDa/217kDa |
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