Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody (AP0824) at 1:1000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-CRKL-Y207 Rabbit pAb
Catalog No:AB-AP0824
Manufacturer:ABclonal
Synonyms:CRKL
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-CRKL-Y207 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
Immunogen: | A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1). |
Gene ID: | 1399 |
Swiss prot: | P46109 |
Molecular weight: | 33kDa |
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