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Western blot analysis of extracts of various cell lines, using Phospho-CRMP2/DPYSL2-T514 antibody (AP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-CRMP2/DPYSL2-T514 Rabbit pAb
Catalog No：AB-AP0821
Manufacturer：ABclonal
Synonyms：DPYSL2;CRMP-2;CRMP2;DHPRP2;DRP-2;DRP2;N2A3;ULIP-2;ULIP2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-CRMP2/DPYSL2-T514 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer’s disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T514 of human CRMP2/DPYSL2 |
| Gene ID: | 1808 |
| Swiss prot: | Q16555 |
| Molecular weight: | 58kDa/62kDa |
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