Western blot analysis of extracts of C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 antibody (AP0814) at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-PTPRA-Y789 Rabbit pAb
Catalog No:AB-AP0814
Manufacturer:ABclonal
Synonyms:PTPRA;HEPTP;HLPR;HPTPA;HPTPalpha;LRP;PTPA;PTPRL2;R-PTP-alpha;RPTPA
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-PTPRA-Y789 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y789 of human PTPRA |
Gene ID: | 5786 |
Swiss prot: | P18433 |
Molecular weight: | 89kDa/90kDa |
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