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Western blot analysis of extracts of Jurkat cells, using Phospho-LAT-Y171 antibody (AP0809) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 ug/mL) for 2 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 5s.
Phospho-LAT-Y171 Rabbit pAb
Catalog No：AB-AP0809
Manufacturer：ABclonal
Synonyms：LAT;LAT1;pp36;IMD52
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-LAT-Y171 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen: | A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1). |
| Gene ID: | 27040 |
| Swiss prot: | O43561 |
| Molecular weight: | 24kDa/27kDa/28kDa |
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