Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 antibody (AP0805) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit pAb
Catalog No:AB-AP0805
Manufacturer:ABclonal
Synonyms:PLCG2;APLAID;FCAS3;PLC-IV;PLC-gamma-2
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. |
Immunogen: | A synthetic phosphorylated peptide around Y1217 of human PLC gamma 2 (PLCG2) (NP_002652.2). |
Gene ID: | 5336 |
Swiss prot: | P16885 |
Molecular weight: | 147kDa |
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