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Western blot analysis of extracts of Jurkat and HeLa cells, using Phospho-PRKCQ-T538 antibody (AP0798) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-PRKCQ-T538 Rabbit pAb
Catalog No：AB-AP0798
Manufacturer：ABclonal
Synonyms：PRKCQ;PRKCT;nPKC-theta
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PRKCQ-T538 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. |
| Immunogen: | A synthetic phosphorylated peptide around T538 of human PRKCQ (NP_006248.1). |
| Gene ID: | 5588 |
| Swiss prot: | Q04759 |
| Molecular weight: | 67kDa/74kDa/81kDa |
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