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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EIF4G1-S1108 antibody (AP0796) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
Phospho-EIF4G1-S1108 Rabbit pAb
Catalog No：AB-AP0796
Manufacturer：ABclonal
Synonyms：EIF4G1;EIF-4G1;EIF4F;EIF4G;EIF4GI;P220;PARK18
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-EIF4G1-S1108 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5′-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. |
| Immunogen: | A synthetic phosphorylated peptide around S1108 of human EIF4G1 (NP_001181875.1). |
| Gene ID: | 1981 |
| Swiss prot: | Q04637 |
| Molecular weight: | 154kDa/158kDa/166kDa/171kDa/175kDa/176kDa |
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