.jpg)
Western blot analysis of extracts of HeLa and C6 cells, using Phospho-PEA15-S104 antibody (AP0788) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of HeLa cells, using Phospho-PEA15-S104 pAb (AP0788) at 1:2000 dilution or PEA15 antibody (A9956).HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-PEA15-S104 Rabbit pAb
Catalog No：AB-AP0788
Manufacturer：ABclonal
Synonyms：PEA15;HMAT1;HUMMAT1H;MAT1;MAT1H;PEA-15;PED
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PEA15-S104 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1). |
| Gene ID: | 8682 |
| Swiss prot: | Q15121 |
| Molecular weight: | 15kDa/17kDa |
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