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Western blot analysis of extracts of HeLa cells, using Phospho-PFKFB2-S483 antibody (AP0784) at 1:2000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-PFKFB2-S483 Rabbit pAb
Catalog No：AB-AP0784
Manufacturer：ABclonal
Synonyms：PFKFB2;PFK-2/FBPase-2
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PFKFB2-S483 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S483 of human PFKFB2 (NP_006203.2). |
| Gene ID: | 5208 |
| Swiss prot: | O60825 |
| Molecular weight: | 54kDa/58kDa |
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