Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Phospho-ACLY-S455 pAb (AP0779). Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 pAb (AP0779) at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody (AP0779) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-ACLY-S455 Rabbit pAb
Catalog No:AB-AP0779
Manufacturer:ABclonal
Synonyms:ACLY;ACL;ATPCL;CLATP
Applications:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-ACLY-S455 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. |
Immunogen: | A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2). |
Gene ID: | 47 |
Swiss prot: | P53396 |
Molecular weight: | 91kDa/119kDa/120kDa |
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