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Western blot analysis of extracts of HeLa cells, using Phospho-EIF4B-S422 antibody (AP0775) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-EIF4B-S422 Rabbit pAb
Catalog No：AB-AP0775
Manufacturer：ABclonal
Synonyms：EIF4B;EIF-4B;PRO1843
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-EIF4B-S422 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5′-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Immunogen: | A synthetic phosphorylated peptide around S422 of human EIF4B (NP_001408.2). |
| Gene ID: | 1975 |
| Swiss prot: | P23588 |
| Molecular weight: | 64kDa/69kDa |
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