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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-GAB1-Y659 antibody (AP0774) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-GAB1-Y659 Rabbit pAb
Catalog No：AB-AP0774
Manufacturer：ABclonal
Synonyms：GAB1;GRB2
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-GAB1-Y659 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around Y659 of human GAB1 (NP_002030.2). |
| Gene ID: | 2549 |
| Swiss prot: | Q13480 |
| Molecular weight: | 76kDa/80kDa |
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