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Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug Phospho-p53-S33 pAb (AP0762). Western blot was performed from the immunoprecipitate using Phospho-p53-S33 pAb (AP0762) at a dilition of 1:1000. HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.
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Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (AP0762) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat liver using Phospho-p53-S33 antibody (AP0762) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using Phospho-p53-S33 antibody (AP0762) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Phospho-p53-S33 antibody (AP0762) at dilution of 1:100 (40x lens).
Phospho-p53-S33 Rabbit pAb
Catalog No：AB-AP0762
Manufacturer：ABclonal
Synonyms：BCC7;LFS1;P53;TRP53;p53;TP53;BMFS5
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p53-S33 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). |
| Immunogen: | A synthetic phosphorylated peptide around S33 of human p53 (NP_000537.3). |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 23-43kDa |
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