Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eIF4E-S209 antibody (AP0747) at 1:500 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-eIF4E-S209 Rabbit pAb
Catalog No:AB-AP0747
Manufacturer:ABclonal
Synonyms:AUTS19;CBP;EIF4E1;EIF4EL1;EIF4F;eIF-4E;EIF4E
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-eIF4E-S209 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5′ end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5′-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S209 of human Phospho-eIF4E-S209 |
Gene ID: | 1977 |
Swiss prot: | P06730 |
Molecular weight: | 25kDa/27kDa/28kDa |
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