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Western blot analysis of extracts of various cell lines, using Phospho-Caveolin-1-Y14 Rabbit pAb (AP0742) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
Phospho-Caveolin-1-Y14 Rabbit pAb
Catalog No：AB-AP0742
Manufacturer：ABclonal
Synonyms：BSCL3;CGL3;LCCNS;MSTP085;PPH3;VIP21;CAV1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Caveolin-1-Y14 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1. |
| Immunogen: | A synthetic phosphorylated peptide around Y14 of human Caveolin-1 (NP_001744.2). |
| Gene ID: | 857 |
| Swiss prot: | Q03135 |
| Molecular weight: | 17kDa/20kDa |
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