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Western blot analysis of extracts of various cell lines, using Phospho-IkBɑ-S32 Rabbit mAb (AP0707) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
Phospho-IκBα-S32 Rabbit mAb
Catalog No：AB-AP0707
Manufacturer：ABclonal
Synonyms：IKBA;MAD-3;NFKBI;IKB alpha;NFKBIA;EDAID2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-I魏B伪-S32 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen: | A synthesized peptide derived from human Phospho-IKB alpha (S32). |
| Gene ID: | 4792 |
| Swiss prot: | P25963 |
| Molecular weight: | 39kDa |
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