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Western blot analysis of extracts of 293T cells, using Phospho-FOXO3A-S253 antibody (AP0684) at 1:2000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-FOXO3A-S253 Rabbit pAb
Catalog No：AB-AP0684
Manufacturer：ABclonal
Synonyms：FOXO3;AF6q21;FKHRL1;FKHRL1P2;FOXO2;FOXO3A;FoxO3a
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-FOXO3A-S253 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. |
| Immunogen: | A synthetic phosphorylated peptide around S253 of human FOXO3A (NP_001446.1). |
| Gene ID: | 2309 |
| Swiss prot: | O43524 |
| Molecular weight: | 48kDa/71kDa |
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