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Western blot analysis of extracts of HepG2 cells, using Phospho-TNK2-Y284 antibody (AP0589) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-TNK2-Y284 Rabbit pAb
Catalog No：AB-AP0589
Manufacturer：ABclonal
Synonyms：TNK2;ACK;ACK-1;ACK1;p21cdc42Hs
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-TNK2-Y284 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. |
| Immunogen: | A synthetic phosphorylated peptide around Y284 of human TNK2 (NP_005772.3). |
| Gene ID: | 10188 |
| Swiss prot: | Q07912 |
| Molecular weight: | 60kDa/114kDa/119kDa |
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