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Western blot analysis of extracts of various cell lines, using Phospho-MAPKAPK2-T334 antibody (AP0588) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-MAPKAPK2-T334 Rabbit pAb
Catalog No：AB-AP0588
Manufacturer：ABclonal
Synonyms：MAPKAPK2;MAPKAP-K2;MK-2;MK2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-MAPKAPK2-T334 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2). |
| Gene ID: | 9261 |
| Swiss prot: | P49137 |
| Molecular weight: | 42kDa/45kDa |
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