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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-KIT-Y719 antibody (AP0586) at 1:500 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-KIT-Y719 Rabbit pAb
Catalog No：AB-AP0586
Manufacturer：ABclonal
Synonyms：KIT;C-Kit;CD117;PBT;SCFR;c-kit
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-KIT-Y719 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y719 of human Kit |
| Gene ID: | 3815 |
| Swiss prot: | P10721 |
| Molecular weight: | 46kDa/109kDa |
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