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Western blot analysis of extracts of various cell lines, using Phospho-Cyclin D1-T286 pAb (AP0574) at 1:1000 dilution.C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
Phospho-Cyclin D1-T286 Rabbit pAb
Catalog No：AB-AP0574
Manufacturer：ABclonal
Synonyms：BCL1;D11S287E;PRAD1;U21B31;CCND1;Cyclin D1;cyclin D1
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Cyclin D1-T286 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. |
| Immunogen: | A synthetic phosphorylated peptide around T286 of human Cyclin D1 (NP_444284.1). |
| Gene ID: | 595 |
| Swiss prot: | P24385 |
| Molecular weight: | 33kDa |
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