Western blot analysis of extracts of Jurkat cells, using Phospho-RB-S608 antibody (AP0570) at 1:2000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-RB-S608 Rabbit pAb
Catalog No:AB-AP0570
Manufacturer:ABclonal
Synonyms:OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-RB-S608 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
Immunogen: | A synthetic phosphorylated peptide around S608 of human RB (NP_000312.2). |
Gene ID: | 5925 |
Swiss prot: | P06400 |
Molecular weight: | 106kDa |
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