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Western blot analysis of extracts of 293T cells, using Phospho-PKA C-alpha (PRKACA)-T197 antibody (AP0557) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
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Western blot analysis of extracts of various cell lines, using Phospho-PKA C-alpha (PRKACA)-T197 pAb (AP0557) at 1:2000 dilution or PKA C-alpha (PRKACA) antibody (A18603).HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PKA C-alpha (PRKACA)-T197 Rabbit pAb
Catalog No：AB-AP0557
Manufacturer：ABclonal
Synonyms：PRKACA;PKACA;PPNAD4
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PKA C-alpha (PRKACA)-T197 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing’s syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. |
| Immunogen: | A synthetic phosphorylated peptide around T197 of human PKA C-alpha (PRKACA) (NP_002721.1). |
| Gene ID: | 5566 |
| Swiss prot: | P17612 |
| Molecular weight: | 39kDa/40kDa |
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