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Western blot analysis of extracts of various cell lines, using Phospho-PAK1-T212 pAb (AP0555) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PAK1-T212 Rabbit pAb
Catalog No：AB-AP0555
Manufacturer：ABclonal
Synonyms：PAK1;PAKalpha
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PAK1-T212 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around T212 of human PAK1 (NP_002567.3). |
| Gene ID: | 5058 |
| Swiss prot: | Q13153 |
| Molecular weight: | 60kDa/61kDa |
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