Western blot analysis of extracts of HeLa cell line, using Phospho-Smad3-T179 antibody (AP0554). The lane on the right is treated with the antigen-specific peptide.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Western blot analysis of extracts of PC-12 cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb (AP0554) at 1:1000 dilution.PC-12 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-Smad3-T179 Rabbit pAb
Catalog No:AB-AP0554
Manufacturer:ABclonal
Synonyms:HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3;SMAD3
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Smad3-T179 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic’ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. |
Immunogen: | A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1). |
Gene ID: | 4088 |
Swiss prot: | P84022 |
Molecular weight: | 25kDa/35kDa/43kDa/48kDa |
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