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Western blot analysis of extracts of 293T cells, using Phospho-IRS1-S636/639 antibody (AP0551) at 1:500 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-IRS1-S636/639 Rabbit pAb
Catalog No：AB-AP0551
Manufacturer：ABclonal
Synonyms：HIRS-1;IRS1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-IRS1-S636/639 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
| Immunogen: | A synthetic phosphorylated peptide around S636 & S639 of human IRS1 (NP_005535.1). |
| Gene ID: | 3667 |
| Swiss prot: | P35568 |
| Molecular weight: | 131kDa |
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