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Western blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody (AP0536) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 ug/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes.Raji cells were treated by PMA/TPA (200nM) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-FAK-Y576/577 Rabbit pAb
Catalog No：AB-AP0536
Manufacturer：ABclonal
Synonyms：FADK;FAK;FAK1;FRNK;PPP1R71;p125FAK;pp125FAK;PTK2
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-FAK-Y576/577 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around Y576 & Y577 of human PTK2 (NP_722560.1). |
| Gene ID: | 5747 |
| Swiss prot: | Q05397 |
| Molecular weight: | 39kDa/48kDa/63kDa/99kDa/114kDa/119kDa/120kDa |
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