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Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS-S1177 antibody (AP0515). SKOV3 cells were treated by EGF.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOS-S1177 antibody (AP0515) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-NOS3-S1177 antibody (AP0515) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-NOS3-S1177 antibody (AP0515) at dilution of 1:200 (40x lens).
Phospho-eNOS-S1177 Rabbit pAb
Catalog No：AB-AP0515
Manufacturer：ABclonal
Synonyms：NOS3;ECNOS;eNOS
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-eNOS-S1177 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| Immunogen: | A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2). |
| Gene ID: | 4846 |
| Swiss prot: | P29474 |
| Molecular weight: | 67kDa/68kDa/133kDa |
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