Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOS-S1177 antibody (AP0515) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
Phospho-eNOS-S1177 Rabbit pAb
Catalog No:AB-AP0515
Manufacturer:ABclonal
Synonyms:NOS3;ECNOS;eNOS
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-eNOS-S1177 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
Immunogen: | A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2). |
Gene ID: | 4846 |
Swiss prot: | P29474 |
Molecular weight: | 67kDa/68kDa/133kDa |
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