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Western blot analysis of extracts of NIH3T3cells, using Phospho-YAP1-S127 antibody (AP0489) at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37℃ for 60 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
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Immunofluorescence analysis of HeLa cells using Phospho-YAP1-S127 Rabbit pAb (AP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Phospho-YAP1-S127 Rabbit pAb (AP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Phospho-YAP1-S127 Rabbit pAb
Catalog No：AB-AP0489
Manufacturer：ABclonal
Synonyms：COB1;YAP;YAP2;YAP65;YKI;YAP1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-YAP1-S127 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen: | A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1). |
| Gene ID: | 10413 |
| Swiss prot: | P46937 |
| Molecular weight: | 36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa |
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