Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SRC Family-Y416 pAb (AP0480) at 1:1000 dilution or SRC antibody ( A18240).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-SRC Family-Y416 Rabbit pAb
Catalog No:AB-AP0480
Manufacturer:ABclonal
Synonyms:ASV;SRC1;THC6;c-SRC;p60-Src;SRC
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-SRC Family-Y416 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family |
Gene ID: | 2534/3055/3932/4067/6714/7525 |
Swiss prot: | P06241/P08631/P06239/P07948/P12931/P07947 |
Molecular weight: | 59kDa/60kDa |
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