Western blot analysis of extracts of HeLa cells, using Phospho-p53-S46 antibody (AP0476) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-p53-S46 Rabbit pAb
Catalog No:AB-AP0476
Manufacturer:ABclonal
Synonyms:BCC7;LFS1;P53;TRP53;p53;TP53;BMFS5
Applications:WB,IP
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-p53-S46 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). |
Immunogen: | A synthetic phosphorylated peptide around S46 of human p53 (NP_000537.3). |
Gene ID: | 7157 |
Swiss prot: | P04637 |
Molecular weight: | 23-43kDa |
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