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Immunohistochemistry of paraffin-embedded mouse testis using Phospho-NF-kB p65/RelA-S536 antibody (AP0475) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of C6 cells, using Phospho-NF-kB p65/RelA-S536 antibody (AP0475) at 1:500 dilution.C6 cells were treated by TNF-ɑ (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-NF-kB p65/RelA-S536 Rabbit pAb
Catalog No：AB-AP0475
Manufacturer：ABclonal
Synonyms：NFKB3;p65;NF-kB p65;RELA;CMCU
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-NF-kB p65/RelA-S536 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S536 of human RelA |
| Gene ID: | 5970 |
| Swiss prot: | Q04206 |
| Molecular weight: | 58kDa/59kDa/60kDa |
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