Western blot analysis of extracts of Jurkat cells, using Phospho-ZAP70-Y493 antibody (AP0468) at 1:1000 dilution.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-ZAP70-Y493 Rabbit pAb
Catalog No:AB-AP0468
Manufacturer:ABclonal
Synonyms:ZAP70;ADMIO2;IMD48;SRK;STCD;STD;TZK;ZAP-70
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €2.550,00
Product Name: | Phospho-ZAP70-Y493 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y493 of human ZAP70 |
Gene ID: | 7535 |
Swiss prot: | P43403 |
Molecular weight: | 35kDa/55kDa/69kDa |
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