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Western blot analysis of extracts of HeLa cells, using Phospho-NOS3-S1177 pAb (AP0421) at 1:1000 dilution or eNOS antibody (A1548).HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Western blot analysis of extracts of Jurkat cells, using Phospho-eNOS-S1177 antibody (AP0421).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-eNOS-S1177 Rabbit pAb
Catalog No：AB-AP0421
Manufacturer：ABclonal
Synonyms：NOS3;ECNOS;eNOS
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-eNOS-S1177 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S1177 of human eNOS |
| Gene ID: | 4846 |
| Swiss prot: | P29474 |
| Molecular weight: | 67kDa/68kDa/133kDa |
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