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Western blot analysis of extracts of K562 cells, using Phospho-JAK2-Y221 antibody (AP0374) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-JAK2-Y221 antibody (AP0374).
Phospho-JAK2-Y221 Rabbit pAb
Catalog No：AB-AP0374
Manufacturer：ABclonal
Synonyms：JAK2;JTK10;THCYT3
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-JAK2-Y221 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y221 of human Phospho-JAK2-Y221 |
| Gene ID: | 3717 |
| Swiss prot: | O60674 |
| Molecular weight: | 130kDa |
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