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Western blot analysis of extracts of various cell lines, using Phospho-FAK-Y397 antibody (AP0302) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Phospho-FAK-Y397 Rabbit pAb
Catalog No：AB-AP0302
Manufacturer：ABclonal
Synonyms：FADK;FAK;FAK1;FRNK;PPP1R71;p125FAK;pp125FAK;PTK2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-FAK-Y397 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around Y397 of human FAK (NP_722560.1). |
| Gene ID: | 5747 |
| Swiss prot: | Q05397 |
| Molecular weight: | 39kDa/48kDa/63kDa/99kDa/114kDa/119kDa/120kDa |
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