Western blot analysis of extracts of C6 cells, using Phospho-VASP-S239 antibody (AP0187) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-VASP-S239 Rabbit pAb
Catalog No:AB-AP0187
Manufacturer:ABclonal
Synonyms:VASP
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-VASP-S239 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S239 of human Phospho-VASP-S239 |
Gene ID: | 7408 |
Swiss prot: | P50552 |
Molecular weight: | 39kDa |
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