Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CFL1-S3 pAb (AP0178) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Phospho-CFL1-S3 Rabbit pAb
Catalog No:AB-AP0178
Manufacturer:ABclonal
Synonyms:CFL1;CFL;HEL-S-15;cofilin;cofilin-1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-CFL1-S3 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S3 of human CFL1 |
Gene ID: | 1072 |
Swiss prot: | P23528 |
Molecular weight: | 18kDa |
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