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Western blot analysis of extracts of 293T cells, using Phospho-FoxO1-S256 antibody (AP0172) .293T were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts of HeLa cells, using Phospho-FoxO1-S256 pAb (AP0172) at 1:1000 dilution or FOXO1 antibody (A13862).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight..|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 2s.
Phospho-FoxO1-S256 Rabbit pAb
Catalog No：AB-AP0172
Manufacturer：ABclonal
Synonyms：FKH1;FKHR;FOXO1A;FOXO1
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-FoxO1-S256 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. |
| Immunogen: | A synthetic phosphorylated peptide around S256 of human FoxO1 (NP_002006.2). |
| Gene ID: | 2308 |
| Swiss prot: | Q12778 |
| Molecular weight: | 69kDa |
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